Brca2
FLAG tag
lamin
FLAG tag at the C-terminus
Source was S2 cells; bait produced from transfected construct; prey produced from endogenous gene.
The addition of a FLAG tag for Brca2 at the C-terminus disrupts the interaction with Lam. Placement of the FLAG tag at the N-terminus does not disrupt this interaction.
GST tag
MBP tag
Fragment named as Brca2-III
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from recombinant fusion protein.
Interaction requires the presence of pds5; treatment with dsRNA against pds5 disrupts the interaction.
Source was nuclear extract of S2 cells; bait produced from transfected construct; prey produced from transfected construct.
Lamin
