Overexpression of ban reduces the expression of a GFP reporter fused to the hid 3\'UTR. This repression is lost upon mutation of ban target sites.

GFP reporter assay

Source was third instar larval wing discs; bait produced from transgenic construct; prey produced from transgenic construct.

ban represses hid at the level of translation; when the two are co-expressed, ban reduces hid protein levels but does not affect hid mRNA levels.

In vitro synthesized miRNAs were injected into embryos before cellularization, and effects on the reporter were assessed at 10 hr AEL.

GFP reporter assay.

Positive control.

2\'O-methyl oligoribonucleotides antisense to ban leads to derepression of the hid GFP reporter.

Source was embryos of transgenic fly line; bait synthesized in vitro; prey produced from transgenic reporter construct.

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

Luciferase reporter assay.

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

Luciferase reporter assay.

Positive control.

Positive control.

luciferase reporter assay

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.