FB2024_04 , released June 25, 2024
Physical Interaction report
Open Close
General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000095591
Interaction Network
Interactions Browser links
snRNA:U2:38ABa network
snf network
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0138511-12.coIP.NB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
snf 

snf

bait

U1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait protein endogenous to extract.

Prey RNA endogenous to extract.

FBrf0210901-13.F
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
snf 

SNF

bait

dU2

prey

fluorescein label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
SLIV
sufficient binding region
GGCCGGUAUUGCAGUACCGCCGGGUCC
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant protein in bacterial system; prey produced and labeled by in vitro transcription.

FBrf0210901-7.FB.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
snf 

SNF

bait

dU2

prey

[32]P label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
SLIV
sufficient binding region
GGCCGGUAUUGCAGUACCGCCGGGUCC
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant protein in bacterial system; prey produced and labeled by in vitro transcription.

FBrf0239188-15.NMR
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
snf 

dSNF

neutral component
snf 

dSNF

neutral component
snf 
snf 

dSNF

neutral component
snf 

dU2

neutral component

dU2

neutral component

dU2

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
snf 
RRM domain
sufficient binding region
aa 1-101
SLIV domain
sufficient binding region
GCGGCCGUAUUGCAGUACCGCGGCC
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system; RNA chemically synthesized.

The snf-U2 snRNA interaction was curated to the products of all 6 identical or nearly identical U2 snRNA genes.

FBrf0239188-29.ITC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

dU2

bait
snf 

dSNF

prey
snf 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
snf 
RRM domain
sufficient binding region
aa 1-96
SLIV domain
sufficient binding region
GCGGCCGUAUUGCAGUACCGCGGCC
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion protein in bacterial system.

Kd = 782nM. The snf-U2 snRNA interaction is stronger when snf is prebound to U2A, lowering the Kd to 117nM. The snf-U2 snRNA interaction was curated to the products of all 6 identical or nearly identical U2 snRNA genes.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)