FlyBase Gene Report: Dmel\OXA1L

General Information

Symbol

Dmel\OXA1L

Species

D. melanogaster

Name

OXA1L mitochondrial inner membrane protein

Annotation Symbol

CG6404

Feature Type

protein_coding_gene

FlyBase ID

FBgn0027615

Gene Model Status

Current

Stock Availability

2 publicly available

Gene Summary

membrane insertase, mediates mitochondrial membrane insertion of cytochrome c oxidase proteins, localizes to cristae and reticular structures isolated in the matrix in addition to the inner boundary membrane, suggesting that it may participate in the formation of vesicle germination-derived cristae P (Interactive Fly, OXA1L )

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

OXA1, BcDNA:GH02220

Key Links

Genomic Location

Cytogenetic map

67F1-67F1

Sequence location

3L:10,884,261..10,886,471 [-]

Recombination map

3-33

RefSeq locus

NT_037436 REGION:10884261..10886471

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (7 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables membrane insertase activity

inferred from sequence or structural similarity with UniProtKB:P39952

(Gene Ontology Curators, 2002-)

inferred from electronic annotation with InterPro:IPR001708

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000276804

(GO Reference Genome Project, 2011-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in mitochondrial proton-transporting ATP synthase complex assembly

inferred from sequence or structural similarity with UniProtKB:P39952

(Gene Ontology Curators, 2002-)

involved_in protein insertion into mitochondrial inner membrane from matrix

inferred from sequence or structural similarity with UniProtKB:P39952

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN000276806

(GO Reference Genome Project, 2011-)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in mitochondrial crista

inferred from mutant phenotype

(Jiang et al., 2020)

located_in mitochondrial inner boundary membrane

inferred from mutant phenotype

(Jiang et al., 2020)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

located_in membrane

inferred from electronic annotation with InterPro:IPR001708

(InterPro Project Members, 2004-)

located_in mitochondrial inner boundary membrane

inferred from sequence or structural similarity with UniProtKB:P39952

(Gene Ontology Curators, 2002-)

is_active_in mitochondrial inner membrane

inferred from biological aspect of ancestor with PANTHER:PTN000276806

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

YIDC/ALB3/OXA1 MEMBRANE PROTEIN INSERTASE FAMILY

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

YIDC/ALB3/OXA1 MEMBRANE PROTEIN INSERTASE FAMILY -: The YidC/Alb3/Oxa1 membrane protein insertase family facilitate membrane protein insertion and folding. In animals and fungi, this family of proteins mediates insertion of proteins from the matrix into the mitochondrial inner membrane. (Adapted from PMID:25947384.)

Summary (Interactive Fly)

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\OXA1L for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9Y171)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.55

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

OXA1L-RA

FBtr0076272

NM_168424

1605

441

OXA1L-RB

FBtr0076271

NM_140160

1815

441

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

OXA1L-PA

FBpp0076001

48.7

441

10.12

FBpp0076000

48.7

441

10.12

Q9Y171

NP_648417

AAN11911

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

441 aa isoforms: OXA1L-PA, OXA1L-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9Y171

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\OXA1L using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

4.31

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic/larval midgut

(Fisher et al., 2012)

spermatogenesis & adult stage | male

testis | restricted

(Vedelek et al., 2018)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in mitochondrial crista

inferred from mutant phenotype

(Jiang et al., 2020)

located_in mitochondrial inner boundary membrane

inferred from mutant phenotype

(Jiang et al., 2020)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\OXA1L in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\OXA1L

View exonic expression by tissue for Dmel\OXA1L

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0027615

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39222/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0027615

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0027615

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0027615

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6404

Flygut - An atlas of the Drosophila adult midgut

FBgn0027615

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\OXA1L

Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 1 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of OXA1L

Transgenic constructs containing regulatory region of OXA1L

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

OXA1L^GD2407

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (2)

Hsap\OXA1L

14 of 14

Yes

Hsap\COX18

4 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (2)

Rnor\Oxa1l

14 of 14

Yes

Rnor\Cox18

4 of 14

Mus musculus (laboratory mouse) (2)

Mmus\Oxa1l

14 of 14

Yes

Mmus\Cox18

3 of 14

Xenopus tropicalis (Western clawed frog) (3)

Xtro\oxa1l

8 of 13

Yes

Xtro\ankrd37

1 of 13

Yes

Xtro\cox18

1 of 13

Danio rerio (Zebrafish) (2)

Drer\oxa1l

14 of 14

Yes

Drer\cox18

3 of 14

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\oxa-1

14 of 14

Yes

Cele\cox-18

4 of 14

Anopheles gambiae (African malaria mosquito) (2)

Agam\AgaP_AGAP005421

12 of 12

Yes

Agam\AgaP_AGAP005624

3 of 12

Arabidopsis thaliana (thale-cress) (12)

Atha\OXA1

11 of 13

Yes

Atha\OXA1L

11 of 13

Yes

Atha\ALB3

4 of 13

Yes

Atha\AT1G65080

4 of 13

Yes

Atha\AT3G44370

4 of 13

Yes

Atha\ALB4

3 of 13

Yes

Atha\AT1G44890

3 of 13

Yes

Atha\AT2G46455

3 of 13

Yes

Atha\AT3G49150

1 of 13

Yes

Atha\AT5G02480

1 of 13

Yes

Atha\FYD

1 of 13

Yes

Atha\SLT1

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\OXA1

12 of 13

Yes

Scer\COX18

3 of 13

Schizosaccharomyces pombe (Fission yeast) (3)

Spom\oxa101

10 of 12

Yes

Spom\oxa102

10 of 12

Yes

Spom\cox18

4 of 12

Escherichia coli (enterobacterium) (1)

Ecol\yidC

7 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:OXA1L. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (1)

CG4942

6 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

encephalopathy, hypotonia and developmental delay, OXA1L-related

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

OXA1L; OXA1L mitochondrial inner membrane protein

14 of 14

601066

COX18; cytochrome c oxidase assembly factor COX18

4 of 14

610428

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

MIST Molecular Interaction Search Tool

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0027615

MIST (protein-protein) - An integrated Molecular Interaction Database

39222

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Protein export

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-33

Cytogenetic map

67F1-67F1

Sequence location

3L:10,884,261..10,886,471 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

67F1-67F1

Limits computationally determined from genome sequence between P{lacW}tna^L6731 and P{PZ}klu⁰⁹⁰³⁶

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-33

(FlyBase, 2016)

3-27.5

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (2)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

83847

y¹ v¹; P{TKO.GS05218}attP40/CyO

VDRC

v108091

P{KK101020}VIE-260B

Genomic Clones (15)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (25)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH02220

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0027615

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0027615

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: BcDNA:GH02220 CG6404

(Zhang, 1999.11)

Source for identity of: OXA1L CG6404

(Thompson et al., 2018)

Nomenclature comments

Etymology

Named 'OXA1L' after the human ortholog.

(Thompson et al., 2018)

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

BcDNA:GH02220

(Caggese et al., 2006)

CG6404

(Denecke et al., 2020, Thompson et al., 2018, Vedelek et al., 2018, Transgenic RNAi Project members, 2017-, Luck et al., 2014, Porcelli et al., 2010, Keleman et al., 2009.8.5, Caggese et al., 2006, Tripoli et al., 2005)

OXA1

(Jiang et al., 2020, Caggese et al., 2006)

OXA1L

(Thompson et al., 2018)

Name Synonyms

Biogenesis protein OXA1

(Tripoli et al., 2005)

Inner membrane protein OXA1L

(Caggese et al., 2006)

OXA1L mitochondrial inner membrane protein

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 39 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

39222

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9Y171

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q9Y171

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9Y171

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0027615

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39222/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0027615

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0027615

FlyMine - An integrated database for Drosophila genomics

FBgn0027615

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG6404

MARRVEL_MODEL - MARRVEL (model organism gene)

39222

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0027615

DroID - A comprehensive database of gene and protein interactions.

FBgn0027615

DRSC - Results frm RNAi screens

FBgn0027615

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0027615

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0027615

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6404

Flygut - An atlas of the Drosophila adult midgut

FBgn0027615

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0027615

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC031655

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Protein export

MIST (protein-protein) - An integrated Molecular Interaction Database

39222

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (28)

The following external sites may use different assemblies or annotations than FlyBase.

4.31

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