FB2024_03 , released June 25, 2024
FB2024_03 , released June 25, 2024
CV Term Report
Help Close All Open All
General Information
Term RMCE donor element ID (Ontology) FBcv:0007043 (FlyBase CV)
Definition An RMCE donor element contains a region of DNA sequence flanked by a pair of target sites for a recombinase or an integrase, which are arranged such that this donor sequence cassette can be used to replace target sequence of a compatible RMCE target element via recombination-mediated cassette exchange (RMCE). For RMCE mediated by a recombinase, the target cassette is flanked by two non-identical recombinase target sites that each carry a different mutation in the spacer sequence of the target site; this renders the two sites incompatible with each other. A compatible RMCE donor cassette is flanked by the same pair of non-identical sites; in the presence of recombinase, a double recombination event between the identical sites on the donor and target sequences results in replacement of the target cassette with the donor cassette. In the case of RMCE mediated by an integrase, the RMCE cassette is flanked by identical integrase target sites, while a compatible RMCE donor cassette is flanked by copies of the orthogonal integrase target site; in the presence of integrase, a double recombination event results in the target cassette being replaced with that of the donor cassette. In this case, if the flanking sites are in inverted orientation, replacement of the target cassette can occur in either direction.[ FlyBase:FBrf0231034 PubMed:21445009 PubMed:7947678 ]
Also Known As "recombination-mediated cassette exchange donor element"
Comment
Links to Extrernal Ontologies
Annotations
Records annotated with this term OR any of its CHILD TERMS
    Results list data from multiple species. Click on a button above and use the 'Filter by species' options on the resulting HitList to retrieve species-specific data.
Records annotated with this exact term (annotations to child terms are NOT included)
No relevant records available
show Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records
 Full annotation statements 
Relevant FlyBase reports
 Constructs
 RMCE donor element (all annotations which use CV term, excluding "NOT" statements)      76
Spanning Tree (Parents/Children)
Only view relationship: 
  experimental tool descriptor
   |__genome engineering tool
       |__RMCE donor element  76 rec.
Spanning Tree View Settings
Parents/Children
View Depth
Show hierarchy levels: for parents, for children
hide Relationships
Is a genome engineering tool
Part of
hide Synonyms & Secondary IDs
Synonyms
  • "recombination-mediated cassette exchange donor element" EXACT
Secondary IDs
hide External Crossreferences & Linkouts