TGCGAACGGACGTGTGGTGCTAGTGTGGTGGACTATACGACTTTTCTATATTTTTAAGGGCCGCGGCAGTAGAGATAGCC TATTTTTCTCGCGACAGAGCGTGTTAAGTAATCAATTGAAATCCAGCGATTGGGTGAATCGAAAGTTCCTCCCCGATTCC CGACTCCTGCGTGCATTTGAAGCACTTACAGCCGCGATTTCCGCACTCGGGTGCACTGTGCTTTTGTTTATACGACCTCC GGTCGCCCCACATTTTCTGTTGAACTTGTGCCGCCGAGCGGTGTTGTCACTGTCCCACCTCCCCCGCAGAAAATCGAAAC TCCCGTACAGTGGGCGTGACCCAGGGCGAAGCCGATAGATAATAACTTTCCACGGGAACTTGAGTGTGCGTAATATCGCC TATAAAGAGTGTTTGGCAACTTGGCGTTCTGGAGTGTCACCCGCAACCAATTCTAATCGTACATACATATATAAGCCAGC TGATTTGGACGTGTGCTACAGCGATTTCATCCCACTGTGCAAGACGGGCGGCAGAAATTCATAAAACAACAGCTAACAAA TTCGACGAAGAGGCATACGCAAAAGAAACGAAAGAGAGAGCGAGCTTATTGTATTTTTGTAATACTCGTCTGCCCAACAA CTACATTTACTGTAATGTGCAGCCTATAATGTAAGATAATGCAATGACACAGGACCAACTCCCAGTAATGGTTGCAATTT AGCCCAGAAGCCACGAATTCACCAACTCAGCAGAGTGATCAGCGTTTGCACTGAAGGCTGCTAATTGAGGACGAGGATCA TAAATCATTATCTGGCGCCAGCGAGGAGGCGAGCACAGCGACAGCAGCGATTGAGTTGCACCACCAGAGACGAATTAATC ATACGCCGCGTTGGTCGTCCCTCCGTCGATACTAAGATATACATATATACAATGCCTGCGAATCCGCCTCCGCACCACGC GCCGCGCACTTGACACGGGGAGCGGCAGAGTAGCCGGAGAAGCCAGTGAGGCCCAGTCTTGTGTTCGATTTGTGTTTGTT ATTTCTTGTTCGAGCTAGCCGGGCACGCTAGTTGGCATCGTTGGCATCGAATCGAAAAGTGTTTGATTTCCGCGAAAACA AAGTACCGCATGTGGCTGCCAGGGCAAAATCTGCTAGCCGGCAGCACAAACTGCAACAACTGCATCTGCAACTGCAACAG CAACAACAGCAACATCACCAGCAACTGCCGCCAGTTGCCGTCAATTATCGCTCAATATATTCTCCGTTGGCCTGTGATAA ACCCCCTAACGTTCTTAATGAACGACCTTCGGTCCAAGTCATTCTATCGTGCAGGCAAACCAACATCTGCAGCATCCGCG TCGGGCGCGACCACCAACGCCAAGTCGTTCGACAAGGGCCAGAACAACAATCCCCGTACTTACAACAACAGCGGCAAGTC GAACAAGGTGTTCCTGCATTACATACCCCGCGATCCGCGCCTAAGAATTTTCAACAAAACCGCCACCCTGAATAAACACT CGAATCGCACCCTGAGCGAGCTAAACATAACTGAAGATCTGTGCAATTATGGTGAATTGATTGGCGGTGGTGCCGAAACA GTGAAACTAGTGCCAGTGAAATCTTAAGAATATATCCGCAAAATCCGCAGATCACCGAATCAATTTATTCGTTAACAAAA GTTGGCTGTTGGCGTGCGGAGAGAGCATTGCCTGGACTACGATCGGCTACGCACTGCAGTTGACCAACAGAGAGAGGGAA TATTTACCTACCGCCTACTTAAGAAAATTTATGCTGAAATCTAATGCAAATTGTGTTCACTTCCAATAATCGCTTGGACA AGAAAGTGCAAATTTTACAAACCTTTTTATAGACGTAGCTGAACCCTTTACACAACAACACGATCACTGGGACTCCGACA TGGCCAAGCTAGCGACGGATCCAAACGCCTCCAACAACAACAGCTGCGAGGCGGTTAACAACAACCACAATGGCCAGAAC CAGAGCCAAAACGGAGGCGCCTCCAATTACCAGGCCCTGCCTCTTACGCCTGCTCCGGCCAACACGCCACTCCACAAGGC CATCAAGCACGACCTCTTCCCGGAGGTCACCTTCTGCAACCTCTCCGTAGAGGAGCTCGCAGATGGGGCTGGACACAGCC GTGTGGTAAGGAGCAGTGTCATCGAACTGGAGGACGGCACCATGACGTGCCTGATGAACGGAAATGGGCAGGTGAAGCGT CGCAAACGCCTGATCTCGAACGAGTCCGGCGACTCGATCGACTCCAGTTCCACGGAGAAGAAAACGCCCAAGATGCCCGA CGGTGGATATGGGTGGGTGGTGGTGTTCGCCTCTCTGGTCGTGTCCCTTATAGCCGACGGACTGAGCTTCTCATTTGGCC TAATAAACGTGGAACTGCTGGAATACTTCGGAGAGTCCACTTCGAAGACCGCCTGGATATCCTCGCTATTCTTCTCCGTG CCCCTGTTGATGGGACCCATCTGGTCCAATCTGGTGGACAAGTACGGCTGCCGAAAGATGACCATCCTCGGCGGAGTGGT CTCTGCCTTTGGGTTTGCTCTGTCTTCGTTTTGCAACTCGATCGAGATGCTCATGGTGACCTTTGGTATCATTTCGGGAC TGGGCCTAGGCATCGGGTATGTGACCGCGGTGGTGTCCATTGCATTCTGGTTTGACAAGAAGCGCACCTTTGCGACCGGC ATCGGAGCATCGGGCACCGGCATCGGGACCTTTGTCTACGCCCGCCTTACTTCCTATCTAATCGAATCGTACGGCTGGCG CGGAGCCACTCTAATCCTTGGCGGCACCATGCTCAACGCATGCGTTTGCGGCGCCCTCATGCGCGATCCCGACTGGCTGA TCGAGGAAAACCGTCTAGAGAGTCGATCCCAAAGCGTCACCACCTTCTCGAACTCCAGTGTCTGCCTGGAGGAAATAAAA AAGCTGCTGGACACCGGCATCACAAAGGAGGCTGTGCTAGACTCGTTGGTGACCAAGAACAACACGGAGGCCAACCAGCA GATTGACGATCCGTTGGACTCCGCTCTCAAGCGCTACCGCAGCGAGATCTTCCTGCCCACCTTCCTGAGCACTCAGGAGC TTGACAGCATCTGCGAGGTTAAGAGCCTGAGCCGACGCTCCCTACGCCACAAGGAGGGTGAGGAGGCGCCGTCACGAGAG AACCTGCTGTCCATGTCCTCAGGAGCAGGTGCCTATCCCCCATCGACGGCCGTTATAGGCTCGCCAGATGATACCCTAAT GGGCGGAATAGCTCACGAGGCGGCAGAGGAAGCAAAGAAGGCCTATCTGGCCTCCATCGAGACGCTGTCTCCATCGGAAA AGCGCTCCACTGGTAGGACTCCCAATGGCTCGCTCCGCTCCTCGGACGAAGGCTACCTTACGCAGAAGCACGCCAGCTCC CGGTACTCGCTTAACGAAAACATCTTCATGGCCAAGCATACAACACCATCAATCTCCAACCTAAAGGTGAATGGCCTGCG ACACAATTCGGTGGACATCTTGAGCGAGGACATGCACTGCTACTACTCGAAGGACGAGACCTTCGCATTGCTGGAGCCGA GCAGGCGCATCAGGCCCACCGTCATCGCCATTCCGGAACAGGAGCAGTCGGTGAACAGCGAGCTGGCCACCCGTCGCGCA CGCCTGGACAGCATCACGGGAATCCGCCGGCTGTCGCGCTCCAAGAAGCCCAGCCACCACCGATCGAACCTCCGCCGCAA CATCTCGATCCGGAACTCCAACTTCCTTAAGGATATGCGCATTCACCGCAACTCGATCCACTACCGCGGGGCCATGCTGA ACACGCACCGCTACCGGCTGCGCGCCTCCTCCTGTCCCAACATCTACCGCAACTCGATGACCACCATCGCCAAAGAGGAG GAAGATACCTGGTATGACAACTTTGTGGACACCATGAAGTCCATCTTTGACTTTTCACTCTTTCTGGACATGAAGTTCGC CCTCTTCAATCTCTCTACATTGTTTTTGTTCATTTGGTTCATTATCCCCTATCTGTACCTTCCCGATTACATGAAGCAAT ACAAGTACGACGTGAGCCAGAGTGCCGAGTTGATTTCGGATGTCGGCATTGCCCAAACGGTGGGCATGATAGGACTTGGC TATCTGGGGGATCTGTCCTGGATGAACATCAATATATGCTACTCGCTGTGCATGCTGGTGTGCGGAGCGTCTGTGTTCTT CATGCCCCTGCTGATTACCAACTACATGGGCCTGATGGTCATGTGCGTTATTTTCGGATTTACGTTCGCCAGCTCCTTCT CGTTTACGCCCAGCATTCTGGTCAGTATTGTGGATCTGGACGATTTCACGTGTGCCTACGGTCTGGTGCTGCTGGTTCAG GGAGTTGGAATGATCGCAGGACCCCCTATTGCAGGCGTCATATACGAGTATACGGGCAGATGGGATGACACCTTCTATTA TGCTGGAATATTTATCGCACTCTCTGGTGTCTGTTCGTATCTGATCGAGTTCTGTGAAAAGAAAGCACCTAAGGAGAGTG ATAGTGATGTCTTAGAGACTAAAAAAGCTCAACTTTTACACTAGGTTACTTACATTGCACTGTTGAACGCCGCAAGTAGA AGGCAACTGGAGGTACTACAAATCCGAACTGTAGCATAATAATGGACTAGCATTAAATGTTAACGAGTTTCTTATGTCAA ATGGTGTTGCAGCCATAAAATCAGAGCAGAATTGTAACTGAAACTAAGCCTATTCCTAAGCTCTAGCCTGTAAGACTATA CTTGACTTGACTAATCGTAGCTAAGTATCAATATACACCTAACATATACTTACCGCACTAGGGCAATTACTAACCGCATC TTTGTCTAGACAAACTGTTCAATTTCAATCAATATCAATGCAGTGCATCACTGCCAGGTGCAAACGGGTCCCTCAATTGT CTTCTTTACAAAATTTAGAAAAATAGTTTTAATTGCCTTACATTTCGTACGAATATTAATTAAAGGGAATACAATAAACA ACAATCTATGTAACGTAAAAAAAAAAAAAAAAAA
Full-length open reading frame fused to a carboxyl-terminal FLAG-HA tag cassette under the control of a metallothionein promoter.
A cDNA sequence encoding each bait molecule was cloned into the pMK33-C-TAP-FLAG-HA-BD vector as a full-length open reading frame, fused to a carboxyl-terminal FLAG-HA tag cassette under the control of a metallothionein promoter. cDNA sequences are derived from previously characterized and publicly available clones created by the Berkeley Drosophila Genome Project; all inserts are full-length sequence-validated.
