FB2024_02 , released April 23, 2024
Clone: Dmel\RP003531242
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General Information
Symbol
Dmel\RP003531242
Species
D. melanogaster
Name
RP003531242
FlyBase ID
FBcl0701216
Feature type
cDNA_clone
Computed gene(s)
Dmel\uex
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
    Library
    RP_cDNA
    • RNA-Seq of D. melanogaster, iso-1 strain, embryo, 5' ends of capped transcripts.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage
      organism

      Comment: 0-24 hr AEL

      (Hoskins et al., 2011)
      Sequence Data of the Insert
      5' Sequence
      Total bases
      212
      NCBI
      GH834932
      5' sequence dataSequence Downloader
      ATTTTTTTAACGTACTAGTAAATAGTACATTTAAGAGAAAAGGAAAAGGTTAGCACAGTATAATTTGAGAGTGGAAAAAA
GAACAGTTTCTCCAGTAAATATAAAAGTAGGTAGTGACAGATGCCAATAAAATACTCCCAGGAGAGAAGTATGAAAAATA
CGTAATCAGTGCATACACATGTGTGAAATACACACTAAATGGGCTGAACTCC
      Library Information (1)
      Library: RP_cDNA
      Description
      Sample preparation
      Protocol

      Total RNA was isolated. Gene-specific 5' RLM-RACE products were generated using the FirstChoice RLM-RACE procedure (Ambion). Primers were designed to target all FlyBase release 5.12 (October 2008) transcript models that overlap 5' ESTs from the RE (FBrf0152058) and LD (FBrf0127297) cDNA libraries. Transcripts of genes expressed in the embryo based on whole-mount RNA in situ hybridization (FBrf0205271) and literature surveys were also targeted. In all, 8570 distinct primer pairs representing 7742 genes were designed. For 1453 RACE reactions that lacked detectable product, second-round PCR conditions were used that including 5 extra amplification cycles.

      (Hoskins et al., 2011)
      Comment

      Clones were not preserved and are not available for distribution.

      Reported As
      Symbol Synonyms
      5' RLM-RACE
      (Hoskins et al., 2011, Hoskins et al., 2011)
      RP
      RP_cDNA
      modENCODE_1040
      modENCODE_1041
      modENCODE_1042
      modENCODE_1043
      modENCODE_1044
      References (2)
      Research paper (1)
      Hoskins et al., 2011, Genome Res. 21(2): 182--192
      Genome-wide analysis of promoter architecture in Drosophila melanogaster. [FBrf0213090]
      Supplementary material (1)
      Hoskins et al., 2011, Genome Res. 21(2):
      Supplemental Material. [FBrf0213251]
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)