FB2024_04 , released June 25, 2024
Clone: Dmel\IP02601
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General Information
Symbol
Dmel\IP02601
Species
D. melanogaster
Name
IP02601
FlyBase ID
FBcl0319092
Feature type
cDNA_clone
Computed gene(s)
Dmel\E(spl)m4-BFM
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
  • Caution: This cDNA clone replaced by FI14216
Library
IP_cDNA
  • IP cDNA library of D. melanogaster, isolated by an inverse-PCR screen (SLIP) from other cDNA libraries.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    embryonic stage
    organism

    Comment: 0-22 hr AEL

    (Rubin et al., 2000)
    larval stage
    organism
    (Rubin et al., 2000)
    pupal stage
    organism

    Comment: early pupae

    (Rubin et al., 2000)
    adult stage
    adult head
    (Rubin et al., 2000)
    Sequence Data of the Insert
    3' Sequence
    Total bases
    855
    NCBI
    EL870526
    3' sequence dataSequence Downloader
    AAACTTAAATATATATTTCAATCCGTAATACTATATACACACGAAATCTTTATCTCAATTACAGCTAAATCGTCTTAATG
AGCCTTCGTCTTCTACTTTTCTTGTCCGGATCTCGCTTGTCCATTTGCAACTGGAATACTACAACTCGTTGTGGATATAT
TCCGTGGTCACCTTGGCCTCGCCCTCGTGGAAACCATTGCAGTCGACTGGATATTTGGCCTCGCAATTGTATGGCGCATT
GATCACACCGAAGAGGGTCCAGAACGGCGTACCGGTTTCCGTGCTAATGAAGATTGCCGCACCCATGCTCCCGATCTTAA
CGATTTTCGGACTAACTCCGGCAATTAGACTGGACGCCGATGTCGGGCGATTCAGTTGGCTCTGCTTGGGCTGGTGGTGC
TGCTGGTATTGGTCGTGCTGCCTCTGCTTCTCCTGCTGATCGAGAGCCGCTGCATTCAGCTGACAGAGCACGTAGCGATC
GCATTGCGGCGACTTGGAGATGTATCTGTAGGCGCGATGGACTTTCAGCACCGGTTCAATAACCTCCAGGTTAAGCGTAT
CCGTTAGCTTGTCGCTGATCTCGGTGGCATTGAACTGAAGTAATCCGCGTGCTTGGCCAAGTTTTAGCAGTTCCTTGGCA
TAGCCCACGGCTGGCTTGACGTACTGCCTCGAATCGATCTTAACGTTCAGATGATGCTTGGCCACATGGTCCAAGAGATT
CGAGATGGTCTCGCCCGGACGGGAGGGATCGAAGTGGGTCTGCTTGGGGTAACCTTGAGACTTCAGCAAGCCGTTAAACA
TGATCTGTGCCGTCTGGAAGTACCTGAGATACACCTCGGGATTGGCCAGGTAGCT
    5' Sequence
    Total bases
    727
    NCBI
    EL870527
    5' sequence dataSequence Downloader
    TACAGTACACATCTCAGCTACACAGCAGCTAAACACAAAATTCCAAAACACATCATCATGTGCCAGAACAAGATCAACAC
CAACAACACCATGACCATCAAATCGAACAAGAAGCTGTCCTACAGCGTGAAGAAACTGCTGCAGAAGATCTTCAAGCAGC
AACAGCGCGTCGAGGAGGAGCAGAACCTCAAGAACGCCCTCAAGGCCAACTCTTTGGAGTCTCTGGAATCCATGGAAAAC
AGCCGCAACGCCGATCTGGAATCCGCCTCCATCTGCGCCTCTCTGGAGTCCTGCGAGAACGAGGCCAACGAGCGTCTCTC
CCAGTCCTGCGAAATTGAGGACTACGACTTCGAGCAACTGCCCACCGTTCCCGTTCACTTCGTCCGCACCGCTCACGGCA
CATTCTTCTGGACCGCCGTCTCCGATTTGCCAGCCGACAACGACCTCGTCGAGCCCCTCTACTGCAGCACCTCCAACGCC
ATCGCTATCCCCCAGGATCGTTGGGTTCAGGCCTAAAAAATATCAGCGTCCTGCAACCTGCATCCATCATCCGGCAACTA
TGTCTTCCATTGGATCTCCAGCTTTAATCAACGTCTTCCGTTAATTTTTGAAACCATTCCACAAAAATTAACACGCAAGC
CGATCAACTCAACTGAAGAGCTTTACAAAAATTGTGATACATTTTACTTAACAAACACAAAAAAATCAAAATATATATAC
AATTTAA
    Library Information (1)
    Library: IP_cDNA
    Description

    Clones isolated by an inverse-PCR screen (SLIP) using a mixture of the GH, LD, LP, and SD cDNA libraries.

    Sample preparation

    Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

    Protocol

    These clones have been isolated a directed screening process called SLIP (Self-Ligation of Inverse PCR Products). Clones isolated from this screen have been PCR-amplified using a mixture of 4 different plasmid cDNA libraries as template. These include the GH, LD, LP, and SD cDNA libraries.

    (FlyBase Curators, 2016-)
    Comment
    Reported As
    Symbol Synonyms
    IP
    IP_cDNA
    References (1)
    FlyBase analysis (1)
    FlyBase Curators, 2016-, Curation of large dataset metadata by FlyBase.
    Curation of large dataset metadata by FlyBase. [FBrf0232540]
    External Crossreferences and Linkouts ( 1 )
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)