FB2024_03 , released April 23, 2024
Clone: Dmel\AT08601
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General Information
Symbol
Dmel\AT08601
Species
D. melanogaster
Name
AT08601
FlyBase ID
FBcl0008267
Feature type
cDNA_clone
Computed gene(s)
Dmel\Vtld, Dmel\CG42529
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
  • chimeric clone
Library
AT_cDNA
  • AT cDNA library of D. melanogaster, Oregon-R strain, adult male, testis and seminal vesicle.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    adult stage | male
    testis
    (Stapleton et al., 2002)
    seminal vesicle
    (Stapleton et al., 2002)
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    1,438
    NCBI
    AY089291
    Full Length sequence dataSequence Downloader
    TTGTCTTTCCGACTCGAAAGTTTTCACCTTTACCCGTCGAACGCTCCTCCGGCAAAAGACAGTCAAAATTCGATGTTTAC
TTGAAATCCAAAGCCCGTGCTTAGAGCGTAGTGGAGTGGAGAGGAGTTGCGGCAATCCTGAGCTCGATCCCGAATCCGAG
GACATAGTGCTGTTGACGGGTTCGATCACTAGATCCCCACATAAACCACCAACTCCATACCCACACGTGCGCCTTGAAGC
CTAGGGGTCGTTGCACTTGCGAACTATGTGACAGTGGCACTACGGAAACAGCGCCAGGGCCGGGTTTACCACTGCGTCTC
CGGAGCACCAGGATCCGGAAACGCAACCTAAGATTAAGACGTCGCTGAACACAGGATCCGAGGACCAACAACCGATCCCC
GGGCGATCAAAAACCCGGCGCCATGGCCATTCACGAGCAGATCGACAACCTGAACATCTGGTGGTTTCCGCGCGACTTCT
GTCAGTCGCGATTCGGGGAGTTCCAGCGGAGCGGTACCAACTCCTGCACCATCATTTCCCTAATACTGGCCGACAAGGTG
GCCAAGGCGGACAGATTCTATCACAGGGTCTCCGATCTGCCGCTGCGGGGATGGGAGCTCTTCGGCAACGCCATTAACGA
CGGAAACAGCGTGTACCACAATGTGATTACAACTAATACGCCGCACGCCAGGAATCTCAACCTCAACATTCCGGATGCTA
TCGCCGCCATTCGGTCGCAGCACAAGATGAACTTCCGGCTGGAGGAGTGGTTTTACACGCACATGGAGGCCGATCCCAGC
AATCCCATGTACAACCGGAACGTTGCTGTGCAGTTGTCGCGGGTTTTCCAGATAACACTCCAGATGTTCCAGCACGCCAG
CGTGCGAAACAATGTACCCACTAACCTCATACCAGACCATTGTGAATTTTATTATCCGCACAATTAGCTCGATTGAGTGT
CAAGCCATATTTTTGTTCACCACTTGCGAGACCGCCAGCGTGGCCCTTTCGCGCATTGAGATCTATTGCCCAGTATATGT
GATGGTGCCGCTGGAGGAGACAGATGATGCGGAAACCATTACCTGCAAGGTGGAGCTCGCTCGTGCGCTTCATTTGCGCC
GTAACATGCACCCGGTTCTTTACACCAAGGATCTCAACGAGTGCAGCTACAGTCCGATTGAGTTTGGAGTGGACTTTATG
AGGAAGAAGGGCTGCTTGGAGGTTGGAGACTTTGTAGTTACCCTGGAGGTGGGCAAAGAGGATAAGGATAACGTGGTCAT
CGGCATAGGAGAGGATGTTTGCATCCTGCGGGCATTTTACGTATCGCCACTCTTTGCCTGCGAGAAGTTTAAGTACGGTG
GATAACTTTACGGCGTGCAGTTGTGCAGATTACACAGTTAATAAACTCTAAATTAAATGTAAAAAAAAAAAAAAAAAA
    Library Information (1)
    Library: AT_cDNA
    Description

    Cloned cDNAs prepared from polyadenylated RNA isolated from adult male testes and seminal vesicles.

    (Stapleton et al., 2002)
    Sample preparation

    Adult male testes and seminal vesicles were hand dissected from 0-3 day old non-isogenic Oregon-R males from a population cage.

    Protocol

    RNA was extracted from dissected testis and poly(A)+ selected twice (kindly provided by J. Pringle and M. Fuller). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; cDNA size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned directly into EcoRI/XhoI-digested pOTB7 plasmid. cDNAs were transformed into DH5-alpha strain (Gibco/BRL), or for AT121nn clones, into DH5-alpha TonA.

    (Stapleton et al., 2002)
    Comment
    Reported As
    Symbol Synonyms
    AT
    (Stapleton et al., 2002)
    AT_cDNA
    References (1)
    Research paper (1)
    Stapleton et al., 2002, Genome Res. 12(8): 1294--1300
    The Drosophila Gene Collection: identification of putative full-length cDNAs for 70% of D. melanogaster genes. [FBrf0152058]
    External Crossreferences and Linkouts ( 2 )
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)