loss of function allele
The luminal domain (LD) is deleted and 3xTag:HA is inserted into endogenous PEK.
A 2902-bp deletion from Chr3R:5461414..5464315 . The deleted sequence is replaced by a 3xHA sequence. It also has silent mutations from “GG” to “AT” at Chr3R:5460966..5460967 to mutate the gRNA PAM sequence.