A loxP-flanked cassette containing a Disc\RFP3xP3.cUa marker and tubulin 3'UTR is inserted into endogenous Sirt6; 'bck' signifies backcrossing to w1118.
The region including the ATG and the first 17 amino acids of Sirt6 were deleted and replaced with a loxP-flanked cassette containing a Disc\RFP3xP3.cUa marker and a tubulin 3'UTR.
Sirt6bck-L2 adults exhibit increased body weight and size; wings are enlarged; mitochondrial (DNA) content is decreased.
Sirt6bck-L2/Sirt6bck-L2 adults show progressive sensitivity to starvation (i.e. in 22-25-days old adults, but not in young adults), as well as a progressive decrease in climbing ability (i.e. in 10-12 and 35-37 days old adults, but not in 3-5 days old adults).