FB2024_04 , released June 25, 2024
Allele: Ncra\QFQF2.lowUTR.LexAop2.hSNAP25.Syx1A.Hsp70UTR.Tag:V5,Tag:NES(HIV-1-Rev)
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General Information
Symbol
Ncra\QFQF2.lowUTR.LexAop2.hSNAP25.Syx1A.Hsp70UTR.Tag:V5,Tag:NES(HIV-1-Rev)
Species
N. crassa
Name
FlyBase ID
FBal0365177
Feature type
allele
Associated gene
Ncra\QF
Associated Insertion(s)
Carried in Construct
PBac{LexAop2-lowUTR.QF2.V5.hSNAP25.HIVNES.Syx1A.Hsp70UTR}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Cachero et al., 2020)
Progenitor genotype
PBac{LexAop2-QF2.V5.hSNAP25.HIVNES.Syx1A.Hsp70UTR}
(Cachero et al., 2020)
Carried in construct
PBac{LexAop2-lowUTR.QF2.V5.hSNAP25.HIVNES.Syx1A.Hsp70UTR}
Cytology
Description

The Syn21 5' UTR present in PBac{LexAop2-QF2.V5.hSNAP25.HIVNES.Syx1A.Hsp70UTR} has been replaced with the canonical Drosophila Kozak sequence CAAA. As a result lexAop regulates expression of a BacTrace sensor construct beginning with the canonical Drosophila Kozak sequence, followed by QF2 fused to amino acids 141-206 from human SNAP25 (flanked by Tag:V5 at the 5' end and Tag:NES(HIV-1-Rev) at the 3' end), followed by full length Syx1A (to target the inactive sensor to the synaptic membrane) and a Hsp70 3' UTR. (GenBank MN658785.)

(Cachero et al., 2020)
Allele components
Component
Use(s)
Regulatory region(s)
lexAop
Encoded product / tool
QF2
Tagged with
Tag:NES(HIV-1-Rev)
Tag:V5
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Ncra\QFQF2.lowUTR.LexAop2.hSNAP25.Syx1A.Hsp70UTR.Tag:V5,Tag:NES(HIV-1-Rev)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)