FB2024_03 , released April 23, 2024
Allele: Cnoc\ChR1::CsubChRCsChrimson.20XUAS.SPARC2-I.tdTomato
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General Information
Symbol
Cnoc\ChR1::Csub\ChRCsChrimson.20XUAS.SPARC2-I.tdTomato
Species
C. noctigama
Name
FlyBase ID
FBal0353098
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
SPARC2-I-CsChrimson::tdTomato
Transgenic product class
Nature of the Allele
Transgenic product class
Progenitor genotype
Cytology
Description

20XUAS regulatory sequences are separated from sequence encoding tdTomato-tagged CsChrimson by a "SPARC2-I" module that comprises: an attP site of 38bp, a STOP cassette, a second attP site (60bp, full-length site), ribozyme sequence (to prevent leaky expression in the absence of phiC31:int) and an attB site. phiC31:int catalyzes irreversible recombination between the attB site and one of the two attP sites; if the attP site upstream of the STOP cassette is utilized, the STOP cassette is excised, permitting expression of tdTomato-tagged CsChrimson, while if the attP site downstream of the STOP cassette is utilized, the STOP cassette is preserved. The "SPARC2-I" module typically results in expression of the effector (in this case tdTomato-tagged CsChrimson) in approximately 15% of cells (recombination of the attB site with the full-length attP downstream of the STOP cassette is more likely than recombination with the shortened attP site upstream of the STOP cassette).

Allele components
Component
Use(s)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Cnoc\ChR1::Csub\ChRCsChrimson.20XUAS.SPARC2-I.tdTomato
Name Synonyms
Secondary FlyBase IDs
    References (5)