FB2024_03 , released June 25, 2024
Allele: Dmel\spict65
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General Information
Symbol
Dmel\spict65
Species
D. melanogaster
Name
FlyBase ID
FBal0325451
Feature type
allele
Associated gene
Dmel\spict
Associated Insertion(s)
Carried in Construct
Key Links
Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Chiang et al., 2017)
Mutagen
CRISPR/Cas9
(Chiang et al., 2017)
Progenitor genotype
Cytology
Description

CRISPR-mediated knockout to delete the 2nd exon of spict results in imprecise repair at the junction resulting in a short, in-frame insertion of AGAAACAGA between CAGAAC-and-ACA, and internal deletion of spict.

(Chiang et al., 2017)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The starvation-induced death of spermatogonial cells (SGs) observed in wild-type testes is significantly reduced in spict65/spictmut mutants, the mutant males also fail to maintain germline stem cell (GSC) number during prolonged starvation and unlike in wild-type controls their GSC numbers steadily decline under starvation. Under fed conditions the number of GSCs in spict65/spictmut mutant testes is comparable to controls.

      In spict65/spictmut mutant testes from starved flies cultured ex-vivo fewer SGs initiate cell death compared to controls and of these only 21% progress beyond the initial phase (level of vas protein decreases as LysoTracker signal begins to appear) of the SG cell death process, suggesting defects in both cell death initiation and progression.

      (Chiang et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      spict65
      (Chiang et al., 2017)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)