FlyBase Allele Report: Scer\GAL4[VGlut.VGN6341]

General Information

Symbol

Scer\GAL4^{VGlut.VGN6341}

Species

S. cerevisiae

Name

FlyBase ID

FBal0324125

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{VGlut-GAL4.VGN6341}

Also Known As

VGN6341-Gal4, vGlut^VGN6341GAL4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Syed et al., 2016)

Progenitor genotype

Carried in construct

P{VGlut-GAL4.VGN6341}

Cytology

Description

505bp of the VGlut enhancer (coordinates BDGP6:2L:2394617:2395172 ) regulate expression of a GAL4 driver.

(Syed et al., 2016)

Allele components

Component

Use(s)

Regulatory region(s)

VGlut

VGlut_VGN6341

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

Additional Information

Statement

Reference

ScerGAL4^{VGlut.VGN6341} drives expression mainly in the adult subsesophageal zone, with expression in ~12 neurons on each side of the adult pars intercerebralis that project into the subesophageal zone. In the adult subsesophageal zone there are two clusters of neurons each side of the midline, one medial and the other lateral. The medial cluster has 6 neurons on each side, 2 of which are motor neurons innervating muscles of the proboscis and the lateral cluster also has 6 neurons on each side, 3 of which are motor neurons innervating muscles of the proboscis, the remaining neurons of each are interneurons, including the bitter gustatory local interneuron. Expression is also seen in neurons, including leg motor neurons, of the thoracico-abdominal ganglion.

(Bohra et al., 2018)

ScerGAL4^{VGlut.VGN6341} drives expression in 3-4 motor neurons that innervate the tibial depressor muscle and long tendon muscle 2 of the femur and 1-2 motor neurons that innervate the tarsal depressor muscle in the tibia.

(Syed et al., 2016)

Marker for

Reflects expression of

VGlut

(Bohra et al., 2018, Syed et al., 2016)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

axon | adult stage, with PlexA^GD2499

(Syed et al., 2016)

axon | adult stage, with PlexA^GD14483

(Syed et al., 2016)

axon | adult stage, with Sema1a^GD2504

(Syed et al., 2016)

axon | adult stage, with Sema1a^GD14307

(Syed et al., 2016)

motor neuron | adult stage, with PlexA^GD2499

(Syed et al., 2016)

motor neuron | adult stage, with PlexA^GD14483

(Syed et al., 2016)

motor neuron | adult stage, with Sema1a^GD2504

(Syed et al., 2016)

motor neuron | adult stage, with Sema1a^GD14307

(Syed et al., 2016)

somatic muscle cell of leg, with PlexA^GD2499

(Syed et al., 2016)

somatic muscle cell of leg, with PlexA^GD14483

(Syed et al., 2016)

somatic muscle cell of leg, with Sema1a^GD2504

(Syed et al., 2016)

somatic muscle cell of leg, with Sema1a^GD14307

(Syed et al., 2016)

Detailed Description

Statement

Reference

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 31[o]C) of Scer\GAL4^{VGlut.VGN6341} neurons in adults results in a significantly reduced proboscis extension reflex in response to tarsal sucrose or water, compared to controls; the reduced response to sucrose is still seen when expression is restricted using Scer\GAL80^tsh-GAL80, but not using Scer\GAL80^ChAT.3.3P.

Inhibition (using shi^1.UAS at 31[o]C) of Scer\GAL4^{VGlut.VGN6341} neurons in adults results in a significantly increased proboscis extension reflex in response to tarsal application of a sucrose and bitter mixture, compared to controls, with no effect on the response to sucrose alone.

(Bohra et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^{VGlut.VGN6341}

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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