FB2024_04 , released June 25, 2024
Allele: Dmel\Gr33aGAL4
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General Information
Symbol
Dmel\Gr33aGAL4
Species
D. melanogaster
Name
FlyBase ID
FBal0243781
Feature type
allele
Associated gene
Dmel\Gr33a
Associated Insertion(s)
TI{GAL4}Gr33aGAL4
Carried in Construct
Also Known As
Gr33a-Gal4
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
recombination
(Moon et al., 2009)
I-SceI
(Moon et al., 2009)
FLPase
(Moon et al., 2009)
ends-out gene targeting
(Moon et al., 2009, Moon et al., 2009)
Progenitor genotype
Associated Insertion(s)
TI{GAL4}Gr33aGAL4
Cytology
Description

Sequence encoding a GAL4 driver has been inserted at the site of the normal translation initiation codon of the Gr33a locus (a mini-w marker has also been inserted in addition to the GAL4 coding region and a portion of the endogenous Gr33a coding region has been replaced by the inserted sequences).

(Moon et al., 2009)
Allele components
Component
Use(s)
Inserted element
TI{GAL4}
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Gr33aGAL4/Gr33aGAL4 or Gr33a1/Gr33aGAL4 mutant males show no difference in courtship intensity as compared with wild type or controls, but lose preference for younger females, instead showing no significant preference for either younger or older females.

      Gr33aGAL4/+ mutant males display no significant differences in courtship intensity or preference for younger females as compared to wild type.

      (Hu et al., 2015)

      Gr33aGAL4 mutant adults display impaired avoidance of umbelliferone in a two-way choice feeding assay compared to wild-type and adult females show impaired umbelliferone avoidance in an assay testing site selection for egg laying.

      (Poudel et al., 2015)

      Mutant flies show normal L-canavanine avoidance in a two-way choice test.

      (Lee et al., 2012)

      Mutant labellar taste bristles show a normal electrophysiological response to 50mM sucrose.

      Mutant flies show a normal behavioural response to sucrose in a two-way choice test (preferring 5mM sucrose to 1mM sucrose).

      Mutant labellar taste bristles show an impaired electrophysiological response to a number of bitter tasting compounds (caffeine, quinine, denatonium, berberine, lobeline, papaverine and strychnine).

      (Moon et al., 2009)

      Mutant labellar taste bristles show an impaired electrophysiological response to a number of bitter tasting compounds (caffeine, quinine, denatonium, berberine, lobeline, papaverine and strychnine).

      Mutant flies show reduced avoidance to a number of bitter compounds (caffeine, quinine, denatonium, berberine, lobeline and papaverine) in a two-way choice assay compared to control flies.

      Gr33a1/Gr33aGAL4 males show increased courtship towards passive, decapitated wild-type males compared to the behaviour of control males in this assay. However, courtship of the mutant males to decapitated or normal females is not significantly different from that of wild type.

      Wild-type males show a normal average time to mating when mated with Gr33a1/Gr33aGAL4 females.

      (Moon et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      References (9)