Mutation of all 16 amino acid residues of the extended G motif (VPVHFVRTAHGTFFWT) to Ala residues.
Expression of m4G1.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4sca-537.4 results in the production of extra bristles (average 36 extra bristles per fly, total of 26 bristle positions on the notum and head assayed).