The expression of Lis-1^KK108813 under the control of Scer\GAL4^shot-OK307 results in shorter and thinner giant fiber terminals and disrupted electrophysiological connection to TTMn (as shown by a severely increased latency and a severely decreased ability to follow stimuli), as compared to controls.

A large fraction of border follicle cells have not initiated migration at stages 9 and 10 in females co-expressing Lis-1^GD1480 and Lis-1^KK108813 under the control of Scer\GAL4^Act.PU in follicle cell clones. The mutant clusters are more rounded than controls at the stage when migration should initiate. The early extensions from the front cell which are normally seen before and at the start of active cluster movement are not rarer in the mutant clusters and do not reach the same size as in controls. The small fraction of mutant border cell clusters that do initiate migration show a greatly reduced net migration speed compared to wild type and the size and frequency of forward cell extensions is severely reduced.

Expression of Lis-1^KK108813 RNAi under the control of Scer\GAL4^GMR.PU result in a significantly increased frequency of axon migration abnormalities in the brain of third instar larvae compared to controls leading to defects in the lamina plexus as well as decreased rhabdomere length in the adult eye.