FlyBase Allele Report: Dmel\foi[20.71]

General Information

Symbol

Dmel\foi^20.71

Species

D. melanogaster

Name

FlyBase ID

FBal0147138

Feature type

allele

Associated gene

Dmel\foi

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Van Doren et al., 2003)

Progenitor genotype

Cytology

Description

Amino acid replacement: ?353term.

(Van Doren et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:8,531,976..8,531,976 [+]

Nucleotide change:

G8531976A

Amino acid change:

W353term | foi-PA; W353term | foi-PB; W353term | foi-PC; W353term | foi-PD

Reported amino acid change:

?353term

Comment:

G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Van Doren et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

germline cell

(Mathews et al., 2006)

gonad | embryonic stage

(Mathews et al., 2006, Mathews et al., 2005)

gonadal sheath proper primordium (with foi^16.33)

(Jenkins et al., 2003)

presumptive embryonic/larval tracheal system

(Mathews et al., 2005)

tracheal lateral trunk primordium

(Van Doren et al., 2003)

Detailed Description

Statement

Reference

foi^20.71 embryos show defective gonad compaction and a failure to ensheath the germ cells.

(Mathews et al., 2006)

In foi^20.71/foi^16.33 mutant embryos gonadal mesoderm and germ cells, but the compaction of gonadal mesoderm is blocked. In addition the gonadal mesoderm cells in these embryos fail to make cellular extensions between and around the germ cells (ensheathment).

(Jenkins et al., 2003)

Mutant embryos show defects in lateral trunk (LT) tracheal branch fusion. This phenotype is highly penetrant; 94% of mutant hemi-embryos have at least one LT fusion defect and 77% have two or more, while the expressivity is incomplete; an average of 3.3 out of 9 LT branches are affected. Thin cellular extensions are sometimes seen between fusion tip cells of failed fusions. Rather than fusing and forming a lumen with the appropriate partners, the defective branches appear to remain independent, but are still capable of extending ganglionic branches ventrally, which is the appropriate behaviour for the properly fused LT branch.

(Van Doren et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

foi[+]/foi^20.71 is an enhancer of abnormal neuroanatomy phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Saja et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

foi^20.71 has gonad | embryonic stage phenotype, suppressible by shg^αTub84B.PP

(Mathews et al., 2006)

Enhancer of

Statement

Reference

foi[+]/foi^20.71 is an enhancer of eye phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Saja et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

A foi^20.71/+ background enhances the visual system degeneration seen in Scer\GAL4^GMR.PF, Ppt1^Scer\UAS.cKa flies.

(Saja et al., 2010)

Expression of shg^αTub84B.PP fully rescues the gonadal phenotype of foi^20.71 mutants in 22% of cases.

(Mathews et al., 2006)

The gonadal mesoderm fails to coalesce in foi^20.71 osk^CE4/foi^16.33 embryos that are derived from osk mutant (foi^20.71 osk^CE4/osk⁸) females.

(Van Doren et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

foi^20.71 is rescued by Scer\GAL4^btl.PS/foi^{531.UAS.Tag:HA}

(Mathews et al., 2005)

foi^20.71 is rescued by Scer\GAL4^twi.PB/foi^{531.UAS.Tag:HA}

(Mathews et al., 2005)

foi^20.71 is rescued by foi^{704.UAS.Tag:HA}/Scer\GAL4^btl.PS

(Mathews et al., 2005)

foi^20.71 is rescued by foi^{704.UAS.Tag:HA}/Scer\GAL4^twi.PB

(Mathews et al., 2005)

foi^20.71 is rescued by foi^{186.UAS.Tag:HA}/Scer\GAL4^btl.PS

(Mathews et al., 2005)

foi^20.71 is rescued by foi^{186.UAS.Tag:HA}/Scer\GAL4^twi.PB

(Mathews et al., 2005)

foi^20.71 is rescued by Scer\GAL4^btl.PS/foi^UAS.cVDa

(Van Doren et al., 2003)

foi^20.71 is rescued by Scer\GAL4^twi.PG/foi^UAS.cVDa

(Van Doren et al., 2003)

foi^20.71 is rescued by foi^{704.UAS.Tag:HA}/Scer\GAL4^twi.PG

(Van Doren et al., 2003)

Partially rescued by

foi^20.71 is partially rescued by foi^{704.UAS.Tag:HA}/Scer\GAL4^twi.PB

(Mathews et al., 2006)

foi^20.71 is partially rescued by Scer\GAL4^twi.PB/foi^{Y646A.UAS.Tag:HA}

(Mathews et al., 2006)

Not rescued by

foi^20.71 is not rescued by foi^{ΔN.UAS.Tag:HA}

(Mathews et al., 2006)

foi^20.71 is not rescued by foi^{D308A.UAS.Tag:HA}

(Mathews et al., 2006)

foi^20.71 is not rescued by foi^{H554A.UAS.Tag:HA}

(Mathews et al., 2006)

foi^20.71 is not rescued by foi^{T557P.UAS.Tag:HA}

(Mathews et al., 2006)

foi^20.71 is not rescued by foi^{E584A.E588A.D591A.UAS.Tag:HA}

(Mathews et al., 2006)

foi^20.71 is not rescued by foi^{H583A.H587A.UAS.Tag:HA}

(Mathews et al., 2006)

foi^20.71 is not rescued by foi^{Catsup.TM6-8.UAS.Tag:HA}

(Mathews et al., 2006)

foi^20.71 is not rescued by foi^{D551A.D558A.UAS.Tag:HA}

(Mathews et al., 2006)

foi^20.71 is not rescued by Scer\GAL4^{VP16.nanos.UTR}/foi^UAS.cVDa

(Van Doren et al., 2003)

Comments

Expression of the foi^{704.Scer\UAS.T:Ivir\HA1} transgene, under the control of Scer\GAL4^twi.PB, rescues the defective gonad compaction and ensheathment phenotype of foi^20.71 mutants.

Expression of the foi^{Y646A.Scer\UAS.T:Ivir\HA1} transgene, under the control of Scer\GAL4^twi.PB, rescues the defective gonad compaction phenotype of foi^20.71 mutants. However, this transgene does not rescue the germ cell ensheathment phenotype displayed by foi^20.71 mutants.

(Mathews et al., 2006)

The gonad coalescence defects seen in foi^20.71 mutant embryos are rescued by expression of foi^{Scer\UAS.cVDa} under the control of Scer\GAL4^twi.PG, but are not rescued by expression of foi^{Scer\UAS.cVDa} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}. The tracheal branch fusion defects seen in foi^20.71 mutant embryos are rescued by expression of foi^{Scer\UAS.cVDa} under the control of Scer\GAL4^twi.PG or Scer\GAL4^btl.PS.

(Van Doren et al., 2003)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

foi^20.713

(Saja et al., 2010)

foi^20.71

(Le Bras and Van Doren, 2006, Mathews et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (7)

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